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KMID : 0616120180490010040
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Korean Journal of Pharmacognosy
2018 Volume.49 No. 1 p.40 ~ p.46
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Antioxidant Activity of n-Butanol Fraction of Chaenomeles sinensis Fruit in Caenorhabditis elegans
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Kim Jun-Hyeong
An Chang-Wan
Noh Yun-Jeong
Kim Su-JIn
Kim Ju-Eun
Abinash Chandra Shrestha
Ham Ha-Neul
Leem Jae-Yoon
Cho Hyoung-Kwon
Kim Dae-Sung
Moon Kwang-Hyun
Lee Jeong-Ho
Jeong Kyung-Ok
Kim Dae-Keun
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Abstract
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Chaenomeles sinensis (Thouin) Koehne fruit (Rosaceae) has been used as a traditional medicine in Korea, Japan and China to treat sore throat, diarrhea and inflammation. The ethanol extract of C. sinensis fruit was successively partitioned as methylene chloride, ethyl acetate, n-butanol and H2O soluble fractions. Among those fractions, the n-butanol fraction showed the most potent DPPH radical scavenging and superoxide quenching activities. To verify antioxidant activities, the n-butanol fraction was checked the activities of superoxide dismutase (SOD) and catalase activities, and intracellular ROS levels and oxidative stress tolerance in Caenorhabditis elegans. Furthermore, to see if increased stress tolerance of worms by treating of the n-butanol fraction was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain. Consequently, the n-butanol fraction elevated SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Moreover, the n-butanol fraction-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.
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KEYWORD
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Chaenomeles sinensis , Caenorhabditis elegans , Antioxidant , SOD , Catalase , ROS
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